University of Toronto
Toronto researchers have discovered that a single molecular
event in our cells could hold the key to how we evolved to become the smartest
animal on the planet.
Benjamin Blencowe, a professor in the University of Toronto's
Donnelly Centre and Banbury Chair in Medical Research, and his team have
uncovered how a small change in a protein called PTBP1 can spur the creation of
neurons -- cells that make the brain -- that could have fuelled the evolution
of mammalian brains to become the largest and most complex among vertebrates.
The study is published in the August 20 issue of Science.
Brain size and complexity vary enormously across vertebrates,
but it is not clear how these differences came about. Humans and frogs, for
example, have been evolving separately for 350 million years and have very
different brain abilities. Yet scientists have shown that they use a remarkably
similar repertoire of genes to build organs in the body.
So how is it that a similar number of genes, that are also switched on or off in similar ways in diverse vertebrate species, generate a vast range of organ size and complexity?
The key lays in the process that Blencowe's group studies, known
as alternative splicing (AS), whereby gene products are assembled into
proteins, which are the building blocks of life. During AS, gene fragments --
called exons -- are shuffled to make different protein shapes. It's like LEGO,
where some fragments can be missing from the final protein shape.
AS enables cells to make more than one protein from a single
gene, so that the total number of different proteins in a cell greatly
surpasses the number of available genes. A cell's ability to regulate protein
diversity at any given time reflects its ability to take on different roles in
the body.
Blencowe's previous work showed that AS prevalence increases with
vertebrate complexity. So although the genes that make bodies of vertebrates might
be similar, the proteins they give rise to are far more diverse in animals such
as mammals, than in birds and frogs.
And nowhere is AS more widespread than in the brain.
"We wanted to see if AS could drive morphological
differences in the brains of different vertebrate species," says Serge
Gueroussov, a graduate student in Blencowe's lab who is the lead author of the
study.
Gueroussov previously helped identify PTBP1 as a protein that takes on
another form in mammals, in addition to the one common to all vertebrates. The
second form of mammalian PTBP1 is shorter because a small fragment is omitted
during AS and does not make it into the final protein shape.
Could this newly acquired, mammalian version of PTBP1 give clues
to how our brains evolved?
PTBP1 is both a target and major regulator of AS. PTBP1's job in
a cell is to stop it from becoming a neuron by holding off AS of hundreds of
other gene products.
Gueroussov showed that in mammalian cells, the presence of the
second, shorter version of PTBP1 unleashes a cascade of AS events, tipping the
scales of protein balance so that a cell becomes a neuron.
What's more, when Gueroussov engineered chicken cells to make
the shorter, mammalian-like, PTBP1, this triggered AS events that are found in
mammals.
"One interesting implication of our work is that this
particular switch between the two versions of PTBP1 could have affected the
timing of when neurons are made in the embryo in a way that creates differences
in morphological complexity and brain size," says Blencowe, who is also a
professor in the Department of Molecular Genetics.
As scientists continue to sift through countless molecular
events occurring in our cells, they'll keep finding clues as to how our bodies
and minds came to be.
"This is the tip of an iceberg in terms of the full
repertoire of AS changes that likely have contributed major roles in driving
evolutionary differences," says Blencowe.
