Barnacles
offer genetic clues on how organisms adapt to changing environments
By Phoebe
Hall, Assistant Director of BioMedical Communications, Division of Biology and
Medicine
Densely settled barnacles are pictured in the intertidal near Fort Wetherill in Jamestown, Rhode Island. |
What genes help organisms survive in
changing environments? As climate change impacts species across the planet,
it’s a big question in basic biology. New research on barnacles may provide
some answers.
Barnacles
are crustaceans, related to shrimps and crabs. After a brief period when they
float freely around the ocean, barnacle larvae attach to a hard surface — a
rock, a boat, a whale — and develop into adults.
They build hard plates surrounding their bodies, which they can open to feed and to reproduce, and close protectively during low tide and other harsh conditions.
They build hard plates surrounding their bodies, which they can open to feed and to reproduce, and close protectively during low tide and other harsh conditions.
How
barnacles not only survive these radically changing habitats, but choose mates
and evolve, has fascinated biologists since the days of Charles Darwin, who
wrote multiple volumes on the subject.
“They make a commitment to an environment,” said David Rand, chair of the Department of Ecology and Evolutionary Biology at Brown University. “If they make the wrong decision, they’re dead. If they make the right decision, they reproduce.”
When
Rand came to Brown in 1991, he wanted to use barnacles to identify the gene or
genes that seem to allow individuals to adapt to high-stress areas — typically
the high intertidal zone, which remains dry for hours between high tides.
Conversely, he hypothesized, barnacles settled in the low intertidal zone, which is usually underwater, would have different forms of ecologically important genes.
Conversely, he hypothesized, barnacles settled in the low intertidal zone, which is usually underwater, would have different forms of ecologically important genes.
“How
do we find a needle in a haystack?” asked Rand, also a professor of natural
history and of biology. “Of tens of thousands of genes in a genome, how do we
find those genes that are meaningful to climate change, environmental
heterogeneity, the Darwinian problem?”
In
the 1990s, Rand and graduate student Paul Schmidt — who earned his Ph.D. from
Brown in 1999 and is now a professor at University of Pennsylvania — used a
technique called classic protein gel electrophoresis to identify a central
metabolic protein, mannose-6 phosphate isomerase (Mpi), that allows the
barnacle Semibalanus balanoides to survive in its variable
habitat. Subsequent researchers in his lab built on that work to identify the
structure of the gene encoding the Mpi protein.
Fast-forward
a couple of decades, and genome sequencing technology has now caught up with
Rand’s original hypothesis — and it’s validated the earlier work. Last month,
the newest study from
Rand’s research team was published in the Proceedings of the National Academy
of Sciences.
Led by Brown graduate student Joaquin Nunez, the team reported molecular evidence of natural selection on the Mpi gene in Semibalanus balanoides, confirming the hypothesis that the gene helps the barnacle to survive the extreme changes of the intertidal zone.
Led by Brown graduate student Joaquin Nunez, the team reported molecular evidence of natural selection on the Mpi gene in Semibalanus balanoides, confirming the hypothesis that the gene helps the barnacle to survive the extreme changes of the intertidal zone.
Nunez,
who joined Rand’s lab nearly five years ago, analyzed the genomic data to tie
it together.
“You
always build upon the knowledge that’s before you,” Nunez said.
The
team showed, at the nucleotide level, that different versions of the Mpi gene
are present at different frequencies depending where the barnacles are found in
the intertidal zone. The enzyme plays an important role in glycolysis, which
converts sugars to energy; one form performs well under high stress, like a hot
day at low tide; the other form does better under low stress.
The
study shows how natural selection enables the barnacles’ survival in this
ever-changing environment by maintaining multiple versions of the gene that
codes for that enzyme.
The
paper also uncovered more information about the structural impact of the gene
variant. Nunez found new positions in the protein that the gene encodes, where
mutations altered the sequence of the protein and, presumably, its function.
Colleagues from Sweden modeled the two versions of the protein, suggesting how they would conform to different environmental stressors. These predicted structures are consistent with the differing Mpi enzyme response to its environment.
Colleagues from Sweden modeled the two versions of the protein, suggesting how they would conform to different environmental stressors. These predicted structures are consistent with the differing Mpi enzyme response to its environment.
Future
research, Rand said, could use structural biology to “help us elucidate the
structures of those alternative forms and get more of the mechanistic details
of how this enzyme works.”
Nunez
said they also found hints that Mpi may interact with other proteins, producing
new characteristics in stressful events — another potential area of study.
Though this paper focuses on the adaptations of this barnacle species, the
bigger picture is the biological system in which the barnacle operates, he
said.
“A
very complex set of things needs to work together for these populations to be
healthy and thrive for millennia,” Nunez said. “One of the big predictions of
climate change is that many natural populations are going to find themselves in
environments that aren’t just hotter or more humid or more stressful, but those
environments are going to experience very strong fluctuations.”
The
intertidal is a “natural laboratory” to understand how species survive and
prosper in fluctuating extremes, Nunez said. The same methods that he and Rand
used to show how barnacle genes adapt to those extremes could be used by
entomologists who want to know how honeybees are adapting to climate change, or
crop scientists concerned about productivity in a more stressful environment.
“There are labs all around the world looking for evolutionary approaches to solve this adaptation to climate change,” Rand said.
In
addition to Rand and Nunez, other authors from Brown were Patrick Flight,
Kimberly Neil, Stephen Rong and David Ferranti. Leif Eriksson, Magnus Alm
Rosenblad and Anders Blomberg from the University of Gothenburg also
co-authored the paper.
The
National Science Foundation (IGERT: DGE-0966060), the National Institutes of
Health (2R01GM067862), the Carl Trygger Foundation (CTS 11:14), and the Swedish
Research Council (2017-04559, 2014-03914) supported the research. Nunez, Neil
and Rong are all NSF Graduate Research Fellows and IGERT Fellows, and Ferranti
was supported by a Brown University UTRA Fellowship.