Carrots are healthy, but active enzyme unlocks full benefits
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Beta-carotene is the bioactive compound that gives carrots their orange color. Studies with humans and mice show the conversion of beta-carotene to vitamin A reduces "bad" cholesterol in the blood.
Thus, beta-carotene can help protect against atherosclerosis development, which leads to the accumulation of fats and cholesterol in our arteries.
Atherosclerosis cardiovascular disease is the
primary cause of death worldwide, says Jaume Amengual, assistant professor of
personalized nutrition in the Department of Food Science and Human Nutrition at
University of Illinois.
Amengual
and his colleagues conducted two studies to further understand the effects of
beta-carotene on cardiovascular health. They confirmed its importance, but
identified a critical step in the process.
Beta-carotene
converts to vitamin A with the help of an enzyme called beta-carotene oxygenase
1 (BCO1). A genetic variation determines if you have a more or less active
version of BCO1. People with a less active enzyme could need other sources for
vitamin A in their diet, Amengual says.
The
first study, published in the Journal of Nutrition, analyzed blood
and DNA samples from 767 healthy young adults aged 18 to 25. As expected the
researchers found a correlation between BCO1 activity and bad cholesterol
level.
"People who had a genetic variant associated with making the enzyme BCO1 more active had lower cholesterol in their blood. That was our first observation," Amengual notes.
To
follow up on these findings, Amengual and his colleagues conducted a second
study, published in the Journal of Lipid Research, using mice.
"In
the human study, we saw that cholesterol was higher in people who do not
produce much vitamin A. To know if that observation has an effect in the long
run, we would have to wait 70 years to see if they develop cardiovascular. In
real life, that is not doable. That's why we use animals for certain studies,
so we can speed up the process," he explains.
"The
main findings of the mice study reproduce what we found in humans. We saw that
when we give beta-carotene to mice, they have lower cholesterol levels. These
mice develop smaller atherosclerosis lesions, or plaques, in their arteries.
This means that mice fed beta-carotene are more protected against
atherosclerosis than those fed a diet without this bioactive compound,"
Amengual states.
In
the second study, the researchers also investigated the biochemical pathways of
these processes, determining where in the body the effect occurs.
"We
narrow it down to the liver as the organ in charge of producing and secreting
lipoproteins to the bloodstream, including those lipoproteins known as bad
cholesterol. We observed that in mice with high levels of vitamin A, the
secretion of lipids into the bloodstream slows down," Amengual notes.
Understanding
how the BCO1 enzyme relates to cholesterol has important implications.
Typically, high beta-carotene levels in the blood are associated with health
benefits. But it could also be a sign of a less active BCO1 enzyme that is not
converting the beta-carotene we eat into vitamin A.
Up
to 50% of the population have the less-active variant of the enzyme, Amengual
notes. That means their body is slower at producing vitamin A from a plant
source, and they could need to get this nutrient directly from an animal source
such as milk, or cheese, for example.
