Scientists uncover how sea lamprey brain development is remarkably similar to that of humans
Stowers Institute for Medical Research
Research from the lab of Investigator Robb Krumlauf,
Ph.D., published on February 20, 2024 in Nature Communications offers
a glimpse into how the brains of ancient animals evolved.
The team unexpectedly uncovered that a crucial molecular
cue is very broadly required during vertebrate hindbrain development.
"This study on the hindbrain is essentially a window into the distant past and serves as a model for understanding the evolution of complexity," said co-author Hugo Parker, Ph.D.
Like other vertebrate animals, sea lampreys have a
backbone and skeleton, but they are noticeably missing a feature of their heads
-- a jaw.
Because most vertebrates, including humans, have jaws,
this striking difference in sea lampreys makes them valuable models for
understanding the evolution of vertebrate traits.
"There was a split at the origin of vertebrates
between jawless and jawed around 500 million years ago," said Alice
Bedois, Ph.D., a former predoctoral researcher in the Krumlauf Lab and lead
author on the study.
"We wanted to understand how the vertebrate brain
evolved and if there was something unique to jawed vertebrates that was lacking
in their jawless relatives."
Previous work from the Krumlauf Lab and the lab of
Marianne Bronner, Ph.D., at the California Institute of Technology had
identified that the genes structuring and subdividing the sea lamprey hindbrain
are identical to those in jawed vertebrates including humans.
However, these genes are part of an interconnected
network or circuit that needs to be initiated and directed to build the
hindbrain correctly.
The new study identified a common molecular cue, while
known to direct head-to-tail patterning in a wide variety of animals, as part
of the gene circuitry guiding hindbrain patterning in sea lampreys.
"We found that not only are the same genes but also
the same cue is involved in sea lamprey hindbrain development, suggesting this
process is ancestral to all vertebrates," said Bedois.
This cue is called retinoic acid, commonly known as
vitamin A. While the researchers knew that retinoic acid cues the gene
circuitry to build the hindbrain in complex species, it was not thought to be
involved for more primitive animals like sea lampreys.
Surprisingly, they found that the sea lamprey core
hindbrain circuit is also initiated by retinoic acid, providing evidence that
these sea monsters and humans are much more closely related than anticipated.
"People thought that because sea lampreys lack a
jaw, their hindbrain was not formed like other vertebrates," said
Krumlauf.
"We have shown that this basic part of the brain is
built in exactly the same way as mice and even humans."
There are well known signaling molecules that inform the
fate of cells during development.
Now, the researchers have found that retinoic acid is
another major player that cues vital steps in development like formation of the
brain stem.
In addition, if hindbrain formation is a conserved
feature for all vertebrates, other mechanisms must be responsible to explain
their incredible diversity.
"We all derived from a common ancestor," said
Bedois. "Sea lampreys have provided an additional clue. Now we need to
look even further back in evolutionary time to discover when the gene circuitry
governing hindbrain formation first evolved."
This work was funded by the National Institute of Neurological Disorders and Stroke (award: R35NS111564) of the National Institutes of Health (NIH) and by institutional support from the Stowers Institute for Medical Research. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.